Effect of Cell Size and Shape on Single-Cell Electroporation
نویسندگان
چکیده
منابع مشابه
Effect of Cell Size and Shape on Electric Field Threshold and Critical Transmembrane Voltage for Electroporation
Introduction: Electroporation is a technique for increasing the permeability of the cell membrane to otherwise non-permeate molecules due to an external electric field. This permeability enhancement is detectable if the induced transmembrane voltage becomes greater than a critical value which depends on the pulse strength threshold. In this study, the variabil...
متن کاملeffect of cell size and shape on electric field threshold and critical transmembrane voltage for electroporation
introduction: electroporation is a technique for increasing the permeability of the cell membrane to otherwise non-permeate molecules due to an external electric field. this permeability enhancement is detectable if the induced transmembrane voltage becomes greater than a critical value which depends on the pulse strength threshold. in this study, the variabil...
متن کاملA Quantitative Study of the Effect of Electroporation on the Electropermeability and Cell Survival
Introduction: Electroporation is a practical technique used to transport the molecules across the cell membrane. The utilization of fluorescent molecules is the method widely used to evaluate the electropermeability of cell membrane as a result of pulse application. It is also possible to use mathematical methods to predict the changes caused in cell electropermeability as a result of the chang...
متن کاملSingle-Cell Electroporation of Neurons.
Single-cell electroporation allows the transfection of a small number of neurons in an organotypic culture with a single plasmid or a defined mixture of plasmids. Desired protein expression levels can vary depending on the experimental goals (e.g., high expression levels are needed for optogenetic experiments); however, when too much protein is expressed, cellular toxicity and cell death may ar...
متن کاملA single cell electroporation chip.
Increasing the cell membrane's permeability can be accomplished via single cell electroporation. Polar substances that cannot otherwise permeate the plasma membrane (such as dyes, drugs, DNA, proteins, peptides, and amino acids) can thus be introduced into the cell. We developed a polymeric chip that can selectively immobilize and locally electroporate single cells. This easy-to-use chip focuse...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2007
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac062049e